Related Experiment Video
Updated: Feb 3, 2026
10:52
Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
Published on: October 14, 2025
665
CRISPRminer is a knowledge base for exploring CRISPR-Cas systems in microbe and phage interactions.
Fan Zhang1, Shijia Zhao2, Chunyan Ren3
1HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology, 150080, Harbin, China.
Communications Biology
|November 6, 2018
Summary
CRISPRminer is a new web tool that analyzes CRISPR-Cas systems, crucial for bacterial immunity and genome editing. It provides comprehensive annotations to advance understanding and applications of these powerful biological systems.
Area of Science:
- Microbiology
- Genomics
- Bioinformatics
Background:
- CRISPR-Cas systems are vital for prokaryotic adaptive immunity.
- These systems have been repurposed as versatile genome editing technologies.
- Understanding their natural functions can inspire novel CRISPR-based tools.
Purpose of the Study:
- To develop CRISPRminer, a comprehensive knowledge base and web server for CRISPR-Cas systems.
- To provide instructive annotations for CRISPR arrays, Cas proteins, and system classifications.
- To enable detection of self-targeting events and inference of microbe-phage interactions.
Main Methods:
- Development of a user-friendly web server.
- Comprehensive data collection and investigation of CRISPR-Cas system knowledge.
- Generation of annotations including CRISPR arrays, Cas proteins, system classification, self-targeting, and anti-CRISPR elements.
Main Results:
- CRISPRminer offers detailed annotations for CRISPR-Cas systems.
- The tool facilitates the classification of CRISPR-Cas systems.
- It aids in identifying self-targeting events and microbe-phage interactions.
Conclusions:
- CRISPRminer is a valuable resource for studying CRISPR-Cas systems.
- The knowledge base supports research into prokaryotic immunity and genome editing.
- The tool is freely accessible to the scientific community.
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