Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Habitat Fragmentation02:31

Habitat Fragmentation

21.4K
Habitat fragmentation describes the division of a more extensive, continuous habitat into smaller, discontinuous areas. Human activities such as land conversion, as well as slower geological processes leading to changes in the physical environment, are the two leading causes of habitat fragmentation. The fragmentation process typically follows the same steps: perforation, dissection, fragmentation, shrinkage, and attrition.
21.4K
Hybridization of Atomic Orbitals I03:24

Hybridization of Atomic Orbitals I

67.3K
The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
67.3K
Hybrid Zones02:29

Hybrid Zones

21.9K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
21.9K
DNA Replication02:40

DNA Replication

59.3K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
59.3K
Hybridization of Atomic Orbitals II03:35

Hybridization of Atomic Orbitals II

49.0K
sp3d and sp3d 2 Hybridization
49.0K
Gas Chromatography: Types of Detectors-I01:21

Gas Chromatography: Types of Detectors-I

1.6K
There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Voltammetric Analysis of Quasi-Reversible Kinetics in Aptamer Self-Assembled Monolayers: Toward Rational E-AB Sensor Design.

ACS sensors·2026
Same author

Revealing the Impact of Phase Transition on n = 1 2D Perovskite Photodetectors With Intrinsically Tunable Narrowband Detection.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

A Modified Methyl Transferase Cofactor to Selectively Silence Gene Expression in Escherichia coli.

Chembiochem : a European journal of chemical biology·2026
Same author

Constructing a Nanopipette-Based DNA Electromechanical Device.

Nano letters·2025
Same author

Quantitative Lateral Flow Assay for Meropenem Determination: A Proof-of-Concept Study.

ACS omega·2025
Same author

Harnessing plasmonic charge dynamics for next-generation battery chemistries.

Chemical communications (Cambridge, England)·2025

Related Experiment Video

Updated: Feb 3, 2026

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.9K

Electric Single-Molecule Hybridization Detector for Short DNA Fragments.

A Y Y Loh1, C H Burgess2, D A Tanase1

  • 1Department of Chemistry , Imperial College London , Exhibition Road , London SW7 2AZ , United Kingdom.

Analytical Chemistry
|November 7, 2018
PubMed
Summary

We developed a new electric sensor using DNA nanotechnology and nanopipets to detect short DNA fragments, like those found in bodily fluids, for disease diagnosis.

More Related Videos

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.3K
Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 5, 2010

28.4K

Related Experiment Videos

Last Updated: Feb 3, 2026

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.9K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.3K
Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 5, 2010

28.4K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Short DNA fragments, including circulating cell-free DNA, are crucial biomarkers for diseases like cancer and infections.
  • Existing methods for detecting these short DNA sequences can be complex and time-consuming.

Purpose of the Study:

  • To develop a novel, label-free electric sensor for the rapid detection of short DNA sequences.
  • To demonstrate the sensor's capability in identifying subtle structural differences in DNA hybridization.
  • To establish a potential diagnostic tool for disease markers found in bodily fluids.

Main Methods:

  • Combining DNA nanotechnology with high-bandwidth single-molecule detection within nanopipets.
  • Utilizing resistive-pulse sensing to analyze DNA hybridization events.
  • Employing an 88-mer target from the RV1910c gene of Mycobacterium tuberculosis as a model system.

Main Results:

  • Demonstrated a statistically robust, label-free hybridization sensor for short DNA sequences (<100 nucleotides).
  • Successfully identified subtle structural differences, such as probe hybridization state, using resistive-pulse sensing.
  • Validated the sensor's effectiveness on a specific gene target associated with tuberculosis antibiotic resistance.

Conclusions:

  • The developed nanopipet-based sensor offers a fast, user-friendly, single-molecule DNA assay technology.
  • This technology has significant potential for multiplexing and high-throughput analysis.
  • The sensor is compatible with point-of-care environments, paving the way for rapid disease diagnostics.