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CCDC102B functions in centrosome linker assembly and centrosome cohesion
Yuqing Xia1, Ning Huang1, Zhiquan Chen1
1Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871, China.
Journal of Cell Science
|November 9, 2018
Summary
Researchers discovered CCDC102B, a new protein crucial for centrosome cohesion and linker assembly. It interacts with key proteins and is regulated during mitosis, clarifying centrosome function.
Area of Science:
- Cell Biology
- Molecular Biology
- Structural Biology
Background:
- The centrosome acts as a microtubule-organizing center (MTOC) in interphase cells, relying on a proteinaceous linker for structural integrity.
- The precise assembly mechanisms of centrosome linker components are not fully understood.
Purpose of the Study:
- To identify novel proteins involved in centrosome linker assembly and understand their role in maintaining centrosome cohesion.
- To elucidate the molecular interactions and regulation of a newly identified centrosome linker protein.
Main Methods:
- Immunofluorescence microscopy to visualize protein localization at the centrosome.
- Co-immunoprecipitation assays to identify protein-protein interactions.
- Phosphorylation analysis and cell cycle progression studies.
Main Results:
- CCDC102B was identified as a novel centrosome linker protein essential for centrosome cohesion.
- CCDC102B is recruited to the centrosome by C-Nap1 and interacts with rootletin and LRRC45.
- CCDC102B facilitates rootletin filament formation and is phosphorylated by Nek2A, leading to its dissociation during mitosis.
Conclusions:
- CCDC102B plays a critical role in the assembly and maintenance of the centrosome linker.
- Understanding CCDC102B function provides new insights into centrosome cohesion and MTOC regulation.
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