Related Experiment Video For Caco-2
Updated: Feb 2, 2026

Using Caco-2 Cells to Study Lipid Transport by the Intestine
Published on: August 20, 2015
A Cell Model of Human Small Intestinal Wall Based on Genetically Modified Caco-2 Cells
A L Rusanov1, E D Luzgina2, I V Vakhrushev3
1Perspektiva Company, Novosibirsk, Russia. alexander.l.rusanov@gmail.com.
Abstract:
We propose a cell model of the human small intestinal wall based on genetically modified Caco-2 cells that allows visualization and quantitative assessment of activation of NF-κB factor and related intracellular pathway by using fluorescence microscopy. A dose-dependent increase in fluorescence intensity of the obtained cells in response to TNFα exposure in concentrations of 1-100 ng/ml was demonstrated. It was found that this parameter correlates with a decrease in the transepithelial resistance of the cell monolayer in response to TNFα and can be used to assess the toxic effects of substances on epithelial cells of the human small intestine.
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