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Updated: Feb 2, 2026

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
Induction of Vasculo- and Osteogenesis in Spheroids Formed by Adipose-Derived Stromal Cells
I N Saburina1,2, A A Gorkun1, A F Fidarov3
1Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
Abstract:
Vascularization of bioengineered bone tissue constructs remains a challenging problem of regenerative medicine. Spheroids generated in 3D culture of adipose-derived stromal cells supplemented with inducing factors demonstrate stable characteristics and express of mesenchymal, endothelial, and osteoblasts markers, and represent a prototype of vascularized microtissue. Adipose-derived stromal cells spheroids induced to both angio- and osteogenic differentiation can be used in development of new innovative technologies for in vitro fabrication of vascularized bioequivalents for repair of large bone defects.
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