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Updated: Feb 2, 2026
11:14
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
Published on: January 6, 2017
8.4K
Sequence and length optimization of membrane active coiled coils for triggered liposome release
Camilla Skyttner1, Robert Selegård1, Jakob Larsson1
1Division of Molecular Physics, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden.
Biochimica Et Biophysica Acta. Biomembranes
|November 14, 2018
Summary
Optimizing peptide-lipid interactions enhances drug delivery. A designed peptide (KVC4) effectively triggered cargo release from liposomes without aggregation, showing promise for tunable drug delivery systems.
Area of Science:
- Biochemistry
- Materials Science
- Drug Delivery
Background:
- Liposome drug delivery systems offer potential but require enhanced efficiency and specificity.
- Tailoring peptide-lipid interactions is crucial for controlled drug release from liposomes.
Purpose of the Study:
- To optimize de novo designed coiled-coil peptides for improved liposome-based drug delivery.
- To investigate the impact of peptide sequence and size on membrane activity and cargo release efficiency.
Main Methods:
- Designed and synthesized coiled-coil peptides with varying sequences and lengths (KVC2-KVC5).
- Covalently conjugated peptides to liposomes and assessed their membrane activity.
- Quantified cargo release efficiency and monitored liposome aggregation.
Main Results:
- Amino acid substitutions enhanced peptide amphipathicity, increasing release fivefold.
- Peptide length significantly influenced membrane interaction and cargo release.
- The KVC4 peptide demonstrated optimal cargo release without inducing liposome aggregation.
Conclusions:
- The KVC4 peptide exhibits ideal properties for triggering tunable liposomal cargo release.
- Optimized peptide design is key for developing advanced bioresponsive drug delivery systems.
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