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Peripheral Nerve Injury Induces Dynamic Changes of Tight Junction Components
Xinghui Wang1, Yang Miao2, Jun Ni3
1Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
Frontiers in Physiology
|November 15, 2018
Summary
Peripheral nerve injury disrupts physical barriers. This study reveals tight junction proteins, including claudins, change dynamically after injury, influenced by matrix metalloproteinases 7 and 9 (MMP7 and MMP9).
Area of Science:
- Neuroscience
- Cell Biology
- Molecular Biology
Background:
- Tight junctions form crucial physical barriers in the peripheral nervous system, regulating flow and protecting the endoneurial microenvironment.
- Nerve injury is known to disrupt these physical barriers, but the specific molecular changes in tight junction components remain unclear.
Purpose of the Study:
- To investigate the dynamic changes of tight junction components following peripheral nerve injury.
- To explore the role of matrix metalloproteinases 7 and 9 (MMP7 and MMP9) in modulating tight junction gene expression after nerve injury.
Main Methods:
- Utilized deep sequencing data and bioinformatic tools to analyze gene expression changes.
- Examined the temporal expression patterns of tight junction pathway components.
- Investigated the in vitro and in vivo effects of MMP7 and MMP9 on tight junction genes.
Main Results:
- Tight junction signaling pathways were found to be activated post-nerve injury.
- Several claudin family members were significantly down-regulated after peripheral nerve injury.
- MMP7 and MMP9 were identified as modulators of claudin 1, claudin 10, and claudin 22 gene expression.
Conclusions:
- Peripheral nerve injury induces dynamic alterations in tight junction components, particularly claudins.
- MMP7 and MMP9 play a role in regulating these changes, impacting key claudin expressions.
- Findings contribute to understanding the molecular mechanisms of peripheral nerve injury and regeneration.
Keywords:
bioinformatic analysismatrix metalloproteinasesperipheral nerve barriersperipheral nerve injurytight junctionMore Related Videos
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