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Updated: Feb 2, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
A fluorescent probe for hypochlorite with colorimetric and fluorometric characteristics and imaging in living cells
Cunjie Hu1, Jianping Li1, Liqiang Yan1
1Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, Guangxi, 541004, PR China.
Abstract:
An excellent fluorescent probe with "turn on" phenomenon for sensitive monitoring hypochlorite (ClO-) was prepared using the mild condensation reaction between coumarin and hydroxylamine (NH2OH). The probe possessed potent selectivity to ClO- with obvious color changes from yellow to light yellow and green to blue fluorescence emission, which could be noticed by the naked eye. Moreover, the probe has been succeeded in imaging ClO- in living A549 cells and thus has the potential prospect in the visual detection of intracellular ClO-.
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