Dipasperoside B, a New Trisiridoid Glucoside from Dipsacus asper

Area of Science:

• Natural Product Chemistry
• Pharmacology
• Immunology

Background:

• Dipsacus asper roots are a source of bioactive compounds.
• Iridoid glucosides represent a class of natural products with diverse biological activities.
• Nitric oxide (NO) plays a crucial role in inflammatory processes.

Purpose of the Study:

• To isolate and characterize novel compounds from Dipsacus asper.
• To investigate the chemical structure of Dipasperoside B.
• To evaluate the inhibitory effect of Dipasperoside B on nitric oxide production.

Main Methods:

• Spectroscopic data analysis (NMR, MS) for structural elucidation.
• Isolation of Dipasperoside B from Dipsacus asper root extract.
• In vitro assays using lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cell line.
• Measurement of nitric oxide production.
• Cytotoxicity assessment.

Main Results:

• Dipasperoside B (1) was identified as a unique naturally occurring iridoid glucoside trimer.
• The structure features an oxaloacetic acid-derived moiety and a secoiridoid nucleus.
• Dipasperoside B exhibited significant inhibition of nitric oxide production (IC₅₀ = 21.3 μM).
• No significant cytotoxicity was observed at effective concentrations.

Conclusions:

• Dipasperoside B is a novel iridoid glucoside trimer with a unique chemical structure.
• Dipasperoside B demonstrates potent inhibitory activity against nitric oxide production.
• This compound warrants further investigation for its therapeutic potential in inflammatory conditions.