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Updated: Feb 2, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
Published on: January 9, 2017
Standard-unit measurement of cellular viability using dynamic light scattering optical coherence microscopy
Julia S Lee1, Kyungsik Eom1, Collin Polucha1
1Center for Biomedical Engineering, School of Engineering, Brown University, Providence, RI, USA.
Dynamic light scattering optical coherence microscopy (DLS-OCM) images intracellular motility (ICM) to assess cell viability. This novel method non-destructively maps diffusion coefficients, correlating with cellular health.
Area of Science:
- Biomedical Optics
- Cellular Imaging
- Biophysics
Background:
- Dynamic Light Scattering Optical Coherence Microscopy (DLS-OCM) integrates DLS and OCM for cellular-resolution 3D mapping of diffusion and flow.
- Current methods for measuring cellular viability are often destructive or lack standardization.
- Diffusion coefficient mapping offers potential for non-destructive, standardized cellular viability assessment but requires validation.
Purpose of the Study:
- To present DLS-OCM imaging of intracellular motility (ICM) as a validated surrogate for non-destructive cellular viability measurement.
- To characterize the relationship between ICM and cellular viability under varying environmental conditions.
Main Methods:
- Simultaneously acquired ICM-contrast DLS-OCM images and calcium fluorescence-contrast images of retinal ganglion cells.
- Manipulated cellular viability by altering environmental conditions (temperature, pH).
- Analyzed diffusion coefficient changes in ICM in response to altered cellular viability.
Main Results:
- DLS-OCM successfully generated ICM-contrast images.
- Measured ICM exhibited consistent changes correlating with manipulated cellular viability.
- Diffusion-coefficient-represented ICM serves as a reliable indicator of cellular health.
Conclusions:
- DLS-OCM imaging of ICM is a promising non-destructive method for assessing cellular viability.
- ICM provides a validated, quantitative measure of cellular health.
- This technique has potential applications in various biological and medical fields requiring cell viability assessment.
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