Analysis of lactase in lactose intolerance supplements

Area of Science:

• Biochemistry
• Enzymology
• Biotechnology

Background:

• Lactose intolerance is a common condition caused by lactase enzyme deficiency.
• Lactase supplements are a primary management strategy for lactose intolerance.
• Lactase serves as an excellent model for advanced enzymatic studies due to its clinical relevance.

Purpose of the Study:

• To develop and implement an upper-level biochemistry laboratory sequence focused on lactase supplements.
• To integrate student inquiry and advanced laboratory techniques in biochemical analysis.
• To compare the protein content and enzymatic activity of different lactase supplements.

Main Methods:

• Bradford assay for protein quantification.
• SDS-PAGE for protein separation and visualization.
• Continuous and discontinuous kinetic assays for enzyme activity measurement.
• Zymography to detect enzyme activity in gels.

Main Results:

• Quantification of protein content across three different lactase supplements.
• Assessment of enzymatic activity profiles for each supplement.
• Comparative analysis of supplement efficacy based on experimental data.

Conclusions:

• The laboratory sequence effectively enhances student understanding of biochemical principles.
• Students gain practical experience with advanced techniques relevant to biochemical research.
• The project provides a safe, cost-effective, and realistic research simulation for biochemistry students.