Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fibril-associated Collagen01:11

Fibril-associated Collagen

3.4K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
3.4K
Self-Evaluation: Self-Enhancement and Self-Verification03:00

Self-Evaluation: Self-Enhancement and Self-Verification

5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
Whole Body Regeneration01:33

Whole Body Regeneration

4.1K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
4.1K
Liver Regeneration01:24

Liver Regeneration

4.3K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
4.3K
Bioavailability Enhancement: Drug Solubility Enhancement01:16

Bioavailability Enhancement: Drug Solubility Enhancement

261
Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
261
Overview of Regeneration and Repair01:19

Overview of Regeneration and Repair

5.2K
Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pediatric extended-release oral formulations: A U.S. market landscape analysis.

Journal of pharmaceutical sciences·2026
Same author

Targeting the NAD<sup>+</sup>-PARP1-XRCC1 axis in ALS.

Trends in molecular medicine·2026
Same author

A nanovaccine induces durable and functional MUC4-targeted T cell responses against pancreatic cancer.

Acta biomaterialia·2026
Same author

Peripheral vaccination-induced brain-resident memory CD8+ T cells durably protect mice against intracranial malignancy.

The Journal of clinical investigation·2026
Same author

The Versatile Role of GDF5 in Chondrogenic Progenitor Cell-mediated Cartilage Regeneration via a Hyaluronic Acid-fibrin IPN Hydrogel Platform.

Pharmaceutical research·2026
Same author

Quantitative Proteomic Profiling of Bronchoalveolar Lavage-Derived Extracellular Vesicles in a Murine Asthma Model.

Chemical research in toxicology·2026

Related Experiment Video

Updated: Feb 2, 2026

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy
05:20

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Published on: June 29, 2021

3.5K

A bioactive collagen membrane that enhances bone regeneration.

Behnoush Khorsand1, Satheesh Elangovan2, Liu Hong3

  • 1Division of Pharmaceutics and Translational Therapeutics, University of Iowa College of Pharmacy, Iowa City, Iowa.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|November 23, 2018
PubMed
Summary

This study enhanced collagen membranes for bone regeneration by incorporating genetic material encoding bone morphogenetic protein-9 (BMP-9). The modified membranes (PCM-pDNA(BMP-9) and PCM-cmRNA(BMP-9)) showed improved bone formation in rat calvarial defects, demonstrating their potential for guided bone regeneration.

Keywords:
RNA therapybone regenerationgene therapymembranes

More Related Videos

Biological Compatibility Profile on Biomaterials for Bone Regeneration
10:28

Biological Compatibility Profile on Biomaterials for Bone Regeneration

Published on: November 16, 2018

13.3K
Use of Human Perivascular Stem Cells for Bone Regeneration
07:05

Use of Human Perivascular Stem Cells for Bone Regeneration

Published on: May 25, 2012

22.0K

Related Experiment Videos

Last Updated: Feb 2, 2026

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy
05:20

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Published on: June 29, 2021

3.5K
Biological Compatibility Profile on Biomaterials for Bone Regeneration
10:28

Biological Compatibility Profile on Biomaterials for Bone Regeneration

Published on: November 16, 2018

13.3K
Use of Human Perivascular Stem Cells for Bone Regeneration
07:05

Use of Human Perivascular Stem Cells for Bone Regeneration

Published on: May 25, 2012

22.0K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Collagen membranes are crucial for guided bone regeneration (GBR).
  • Enhancing membrane bioactivity can improve GBR outcomes.
  • Periosteum's regenerative potential can be harnessed through membrane modifications.

Purpose of the Study:

  • To enhance collagen membranes' bioactivity by incorporating plasmid DNA (pDNA) or chemically modified RNA (cmRNA) encoding bone morphogenetic protein-9 (BMP-9).
  • To evaluate the in vitro and in vivo efficacy of these modified membranes for bone regeneration.

Main Methods:

  • Nanoplexes of polyethylenimine (PEI)-nucleic acids (PEI-pDNA or PEI-cmRNA encoding BMP-9) were incorporated into perforated collagen membranes (PCM).
  • Biodegradation kinetics were assessed in simulated body fluid.
  • In vitro bioactivity was evaluated using bone marrow stromal cells (BMSCs), measuring alkaline phosphatase activity and calcium deposition.
  • In vivo efficacy was tested in a rat calvarial defect model, analyzing bone regeneration via micro-computed tomography and histology.

Main Results:

  • Incorporation of pDNA or cmRNA did not alter the biodegradation profile of PCMs.
  • Alkaline phosphatase activity and calcium deposition showed positive trends in BMSCs exposed to modified PCMs compared to controls.
  • PCM-cmRNA(BMP-9) significantly increased bone volume/total volume % in calvarial defects compared to empty defects.

Conclusions:

  • Resorbable perforated collagen membranes can efficiently deliver pDNA and cmRNA.
  • The delivered pDNA and cmRNA encoding BMP-9 are functional both in vitro and in vivo.
  • Modified collagen membranes show promise for enhancing guided bone regeneration.