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Genome-wide polyadenylation site mapping datasets in the rice blast fungus Magnaporthe oryzae

Marco Marconi1, Ane Sesma1, Julio Luis Rodríguez-Romero1

  • 1Centro de Biotecnología y Genómica de Plantas UPM - INIA, Madrid, Spain.

Scientific Data
|November 28, 2018
PubMed

Insights

Investigating polyadenylation in Magnaporthe oryzae reveals how the Rpb35 protein impacts gene regulation and fungal virulence. This study analyzes polyadenylation site usage in wild-type and mutant strains under various nutrient conditions.

Area of Science:

  • Molecular Biology
  • Mycology
  • Bioinformatics

Background:

  • Polyadenylation is crucial for gene regulation and impacts diverse biological processes.
  • The cleavage factor I protein Rpb35 is essential for pre-mRNA polyadenylation and virulence in the rice blast fungus, Magnaporthe oryzae.

Purpose of the Study:

  • To conduct a global survey of polyadenylation site usage in Magnaporthe oryzae.
  • To compare polyadenylation patterns between wild-type and Δrbp35 mutant strains.
  • To investigate the influence of nutrient conditions on polyadenylation site usage.

Main Methods:

  • Bioinformatic analysis of polyadenylation site usage.
  • Comparative genomics of wild-type and mutant fungal strains.
  • Culturing fungi under simulated infection-related nutrient conditions.

Main Results:

  • Identification of global polyadenylation site usage patterns in M. oryzae.
  • Characterization of differences in polyadenylation between wild-type and Δrbp35 strains.
  • Observation of nutrient-dependent variations in polyadenylation site selection.

Conclusions:

  • Rpb35 plays a significant role in regulating polyadenylation site usage in M. oryzae.
  • Environmental nutrient availability influences polyadenylation dynamics in this fungus.
  • Understanding these mechanisms can inform strategies against rice blast disease.

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