New antiradical capacity assay with the use potentiometric method

Area of Science:

• Electrochemistry
• Oxidative Stress Research
• Antioxidant Analysis

Background:

• Peroxyl radicals are key intermediates in oxidation reactions.
• Understanding antioxidant interactions with radicals is crucial for health and material science.
• Existing methods for antioxidant capacity assessment have limitations.

Purpose of the Study:

• To investigate the dependence of redox potential changes on peroxyl radical generation and antioxidant interactions.
• To develop a direct, original potentiometric method for evaluating antioxidant properties.
• To determine key parameters like induction periods, inhibition coefficients, and antiradical capacity.

Main Methods:

• Utilized a potentiometric method to monitor redox potential changes in real-time.
• Studied the oxidation of antioxidants by initiating peroxyl radicals, involving electron transfer.
• Applied the developed method to quantify antioxidant parameters for various compounds and a herbal extract.

Main Results:

• Demonstrated a regular redox potential change correlating with antioxidant oxidation and electron transfer.
• Successfully determined induction periods and inhibition coefficients for OH, NH, and SH-containing antioxidants.
• Showcased the antiradical capacity of a herbal extract with high correlation to the DPPH assay.
• Achieved relative standard deviation (RSD) below 7%.

Conclusions:

• The proposed potentiometric method offers a direct and reliable approach for assessing antioxidant activity.
• This technique provides valuable insights into the mechanism of antioxidant-radical interactions.
• The method shows promise for accurate and efficient evaluation of antioxidant capacity in diverse samples.