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Prototype of a DNA Vaccine against Marburg Virus
N V Volkova1, O V Pyankov2, A V Ivanova2
1State Research Centre of Virology and Biotechnology "Vector", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia. tasha_wolkowa11.93@mail.ru.
Abstract:
The preparation and study of the biological properties of the pVAKS-GPVM DNA immunogen containing a gene encoding Marburgvirus glycoprotein are described. The specificity of blood serum antibodies of guinea pigs immunized with DNA immunogen was analyzed by ELISA. Inactivated viral preparation, recombinant glycoprotein (GP) obtained in the prokaryotic system and virus-like particles based on the recombinant vesicular stomatitis virus exhibiting Marburgvirus GP were used as the antigens. The neutralizing activity of antibodies of immunized animals was tested in vitro using a pseudovirus system. It was demonstrated that the developed immunogen administered to guinea pigs induced the production of specific antibodies that neutralize virus-like particles and Marburgvirus in cultured Vero cells.
Insights
A novel DNA immunogen encoding Marburgvirus glycoprotein successfully induced specific antibodies in guinea pigs. These antibodies demonstrated the ability to neutralize both virus-like particles and the Marburgvirus in cell cultures.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Marburgvirus poses a significant biosecurity threat.
- Development of effective vaccines and immunogens is crucial for Marburgvirus control.
- DNA immunogens offer a promising platform for vaccine development.
Purpose of the Study:
- To prepare and characterize a DNA immunogen (pVAKS-GPVM) encoding the Marburgvirus glycoprotein (GP).
- To evaluate the biological properties and immunogenicity of the developed DNA immunogen in guinea pigs.
- To assess the specificity and neutralizing activity of antibodies induced by the DNA immunogen.
Main Methods:
- DNA immunogen preparation and administration to guinea pigs.
- Enzyme-linked immunosorbent assay (ELISA) for antibody specificity analysis.
- In vitro neutralization assays using pseudovirus systems and Marburgvirus in Vero cells.
- Antigen characterization using inactivated virus, recombinant GP, and virus-like particles.
Main Results:
- Immunization with pVAKS-GPVM induced specific antibodies in guinea pig serum.
- Antibodies recognized Marburgvirus GP presented in various forms (inactivated virus, recombinant protein, virus-like particles).
- The induced antibodies exhibited neutralizing activity against Marburgvirus in vitro.
Conclusions:
- The pVAKS-GPVM DNA immunogen is effective in eliciting a specific antibody response against Marburgvirus glycoprotein.
- The generated antibodies possess neutralizing capabilities, suggesting potential for Marburgvirus prophylaxis.
- This study supports the potential of DNA-based immunogens for Marburgvirus vaccine development.
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