Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

4.5K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.5K
Archival Research01:40

Archival Research

17.1K
Some researchers gain access to large amounts of data without interacting with a single research participant. Instead, they use existing records to answer various research questions. This type of research approach is known as archival research. Archival research relies on looking at past records or data sets to look for interesting patterns or relationships. For example, a researcher might access the academic records of all individuals who enrolled in college within the past ten years and...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A RiboCancer cell line panel reveals that CLL-associated Rps15 mutations translationally rewire transcription through codon-specific tRNA accommodation defects.

HemaSphere·2026
Same author

Expanding the human proteome with microproteins and peptideins.

Nature·2026
Same author

An expanded reference catalog of translated open reading frames for biomedical research.

Nucleic acids research·2026
Same author

Whole genome sequencing in animal health: applications, challenges, and future directions.

BMC veterinary research·2026
Same author

Dual lipid modulation overcomes ferroptosis resistance in high-risk neuroblastoma.

Cell death and differentiation·2025
Same author

Nanopore sequencing enables tissue-of-origin and pathogen detection in plasma cell-free DNA from critically ill patients.

Cell death discovery·2025
Same journal

Protein Sequence Analysis Using the MPI Bioinformatics Toolkit.

Current protocols in bioinformatics·2020
Same journal

Exploring Manually Curated Annotations of Intrinsically Disordered Proteins with DisProt.

Current protocols in bioinformatics·2020
Same journal

Network Building with the Cytoscape BioGateway App Explained in Five Use Cases.

Current protocols in bioinformatics·2020
Same journal

Expanding the Perseus Software for Omics Data Analysis With Custom Plugins.

Current protocols in bioinformatics·2020
Same journal

Exploring Non-Coding RNAs in RNAcentral.

Current protocols in bioinformatics·2020
Same journal

How to Illuminate the Dark Proteome Using the Multi-omic OpenProt Resource.

Current protocols in bioinformatics·2020
See all related articles

Related Experiment Video

Updated: Feb 2, 2026

The ITS2 Database
16:17

The ITS2 Database

Published on: March 12, 2012

32.3K

Using the sORFs.Org Database.

Volodimir Olexiouk1, Gerben Menschaert1

  • 1Department of Mathematical Modelling, Statistics and Bioinformatics, Universiteit Gent Faculteit Bio-Ingenieurswetenschappen, Gent, Belgium.

Current Protocols in Bioinformatics
|November 29, 2018
PubMed
Summary
This summary is machine-generated.

Ribosome profiling reveals numerous small open reading frames (sORFs) with functional roles. The sORFs.org repository offers a vast collection of these sORFs and tools to analyze their coding potential.

Keywords:
SEPmicropeptideribosome profilingsORFsmall open reading frame

More Related Videos

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research
11:18

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

Published on: January 22, 2011

16.5K
Databases to Efficiently Manage Medium Sized, Low Velocity, Multidimensional Data in Tissue Engineering
09:43

Databases to Efficiently Manage Medium Sized, Low Velocity, Multidimensional Data in Tissue Engineering

Published on: November 22, 2019

6.8K

Related Experiment Videos

Last Updated: Feb 2, 2026

The ITS2 Database
16:17

The ITS2 Database

Published on: March 12, 2012

32.3K
Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research
11:18

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

Published on: January 22, 2011

16.5K
Databases to Efficiently Manage Medium Sized, Low Velocity, Multidimensional Data in Tissue Engineering
09:43

Databases to Efficiently Manage Medium Sized, Low Velocity, Multidimensional Data in Tissue Engineering

Published on: November 22, 2019

6.8K

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Ribosome profiling is a technique for genome-wide mapping of actively translated RNA.
  • Numerous small open reading frames (sORFs) have been identified using ribosome profiling.
  • This has challenged the notion that sORFs are non-functional, revealing important translation products.

Purpose of the Study:

  • To establish a comprehensive public repository of sORFs identified by ribosome profiling.
  • To provide tools and metrics for assessing the coding potential of sORFs.
  • To integrate multi-omics data, including mass spectrometry, to validate sORF-encoded polypeptides.

Main Methods:

  • Utilized ribosome profiling data from 78 datasets across six species.
  • Compiled a database of over 3 million sORFs.
  • Developed a pipeline to rescan mass spectrometry datasets for experimental evidence of sORF translation.
  • Implemented distinct query interfaces, export functionality, and visualization tools.

Main Results:

  • Created sORFs.org, a public repository containing over 3 million sORFs.
  • Provided tools for assessing sORF coding potential and integrated multi-omics data.
  • Enabled systematic identification of sORF-encoded polypeptides through mass spectrometry data analysis.

Conclusions:

  • sORFs.org serves as a valuable resource for researchers studying small open reading frames.
  • The repository facilitates the exploration and validation of functional sORFs and their products.
  • The integrated approach highlights the significant role of sORFs in cellular processes.