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New Approaches for Genome Assembly and Scaffolding
Edward S Rice1, Richard E Green1,2
1Department of Biomolecular Engineering, University of California, Santa Cruz, California 95064, USA; email: esrice@soe.ucsc.edu , ed@soe.ucsc.edu.
Annual Review of Animal Biosciences
|November 29, 2018
Summary
New DNA sequencing technologies enable cost-effective, chromosome-scale genome assembly. These advancements overcome limitations of short-read sequencing, producing more contiguous and complete genome assemblies efficiently.
Area of Science:
- Genomics
- Bioinformatics
- Molecular Biology
Background:
- High-throughput DNA sequencing has increased genome assembly speed but reduced contiguity.
- Traditional genome assembly methods often lack scaffolding data, resulting in fragmented assemblies.
- Previous genome projects relied on clone maps, which are time-consuming and expensive.
Purpose of the Study:
- To provide an overview of chromosome-scale genome assembly challenges.
- To review traditional and novel methods for achieving chromosome-scale assemblies.
- To highlight recent advancements in cost-effective, contiguous genome assembly.
Main Methods:
- Review of existing literature on genome assembly techniques.
- Analysis of new technologies enabling chromosome-scale assembly.
- Case studies of recent genome projects utilizing novel methods.
Main Results:
- Short-read sequencing limits genome assembly contiguity compared to older methods.
- Emerging technologies facilitate high-quality, chromosome-scale assemblies at reduced costs.
- Recent projects demonstrate the successful application of new technologies for contiguous genome construction.
Conclusions:
- Novel technologies are revolutionizing chromosome-scale genome assembly.
- Cost-effective and faster methods are now available for generating highly contiguous genomes.
- These advancements promise to accelerate genomic research and applications.
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