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Updated: Feb 1, 2026

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Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination

Stefan Nagl1

  • 1Department of Chemistry, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR, China. chnagl@ust.hk.

Methods in Molecular Biology (Clifton, N.J.)
|November 30, 2018
PubMed
Summary

This study introduces microfluidic free-flow isoelectric focusing (FFIEF) for continuous protein and peptide separation. The novel method integrates optical pH monitoring for precise biomolecule analysis.

Keywords:
Fluorescent pH sensorFree-flow electrophoresisIsoelectric focusingIsoelectric point determinationMultispectral imaging

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