Related Experiment Video
Updated: Feb 1, 2026
05:26
Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
Published on: April 29, 2020
6.7K
Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications
Cyro L S Chagas1, Thiago M G Cardoso1, Wendell K T Coltro2
1Institute of Chemistry, Federal University of Goias, Goiania, GO, Brazil.
Methods in Molecular Biology (Clifton, N.J.)
|November 30, 2018
Summary
This study developed low-cost paper-based microchip electrophoresis (pME) devices for rapid separation of clinical compounds. The pME-capacitively coupled contactless conductivity detection (C4D) system offers a robust platform for diagnosing renal disorders.
Area of Science:
- Analytical Chemistry
- Biomedical Engineering
- Materials Science
Background:
- Paper-based microfluidic devices offer low-cost, portable solutions for diagnostics.
- Existing methods for separating clinically relevant compounds can be complex and expensive.
- Need for accessible diagnostic tools for renal disorders like diabetes and heart disease.
Purpose of the Study:
- To develop and characterize paper-based microchip electrophoresis (pME) devices.
- To integrate capacitively coupled contactless conductivity detection (C4D) for analysis.
- To demonstrate the device's utility in separating clinically relevant biomarkers.
Main Methods:
- Fabrication of pME devices using laser cutting and thermal lamination.
- Integration of hand-drawn pencil electrodes for C4D.
- Electrophoretic separation of Bovine Serum Albumin (BSA) and creatinine.
Main Results:
- Successfully fabricated cost-effective (<$0.10) and robust pME-C4D devices.
- Achieved rapid separation of BSA and creatinine in under 150 seconds with baseline resolution.
- Demonstrated the device's suitability for analyzing renal disorder biomarkers.
Conclusions:
- Paper-based microchip electrophoresis with C4D is a viable, low-cost diagnostic platform.
- The developed pME-C4D device offers a simple, efficient method for biomarker separation.
- This technology presents a promising alternative for point-of-care diagnostics of renal diseases.
Related Concept Videos
01:30Capillary Electrophoresis: Applications
1.4K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.4K
01:08Power
13.0K
The concept of work involves force and displacement; meanwhile, the work-energy theorem relates the net work done on a body to the difference in its kinetic energy, calculated between two points on its trajectory. While none of these quantities or relations involves time explicitly, we know that the time available to accomplish work is often just as important as the amount of work itself. For example, sprinters in a race may have achieved the same velocity at the finish, therefore,...
13.0K
01:20Electrophoresis: Overview
4.0K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
4.0K
01:22Instantaneous Power
918
Instantaneous power is important in electrical circuits, mainly when dealing with sinusoidal input. Instantaneous power, denoted as p(t), results from the multiplication of the instantaneous voltage (v(t)) across an element and the instantaneous current (i(t)) flowing through it. This relationship adheres to the passive sign convention and represents a fundamental principle in electrical engineering.
918
01:14Complex Power
916
Power engineers have introduced the concept of complex power to determine the cumulative effect of parallel loads. This idea plays a crucial role in power analysis because it encompasses all the details related to the power consumed by a specific load.
Complex power is defined as the multiplication of the voltage and the complex conjugate of the current. The magnitude of this power, known as apparent power, is measured in volt-amperes (VA). Notably, the angle of the complex power equates to the...
Complex power is defined as the multiplication of the voltage and the complex conjugate of the current. The magnitude of this power, known as apparent power, is measured in volt-amperes (VA). Notably, the angle of the complex power equates to the...
916
01:07Electrical Power
3.8K
Electric power is the product of current and voltage, represented in units of joules per second, or watts. For example, cars often have one or more auxiliary power outlets with which you can charge a cell phone or other electronic devices. These outlets may be rated at 20 amps and 12 volts, so that the circuit can deliver a maximum power of 240 watts. Consider a 25 Watt bulb and a 60 Watt bulb. The conversion of electrical energy produces heat and light, while the kinetic energy lost by the...
3.8K