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[Screening efficient constitutive promoters in Corynebacterium glutamicum based on time-series transcriptome
Yingchun Wang1,2,3, Jiao Liu2,3, Xiaomeng Ni2,3
1School of Biological Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|December 1, 2018
Summary
Researchers identified three strong, universally applicable constitutive promoters in Corynebacterium glutamicum, a key industrial microorganism. The PcysK promoter demonstrated superior strength, offering a valuable tool for metabolic engineering and enhancing amino acid production.
Area of Science:
- Microbiology
- Metabolic Engineering
- Synthetic Biology
Background:
- Corynebacterium glutamicum is a vital industrial microorganism for amino acid production.
- Strong constitutive promoters are scarce for C. glutamicum, limiting metabolic engineering applications.
Purpose of the Study:
- To identify and characterize novel strong constitutive promoters for C. glutamicum.
- To evaluate promoter universality across different C. glutamicum strains.
Main Methods:
- Time-series transcriptome analysis using RNA-Seq on a glutamate-producing strain.
- Cloning and evaluation of candidate promoter sequences using red fluorescent protein and LacZ reporter systems.
- Testing promoter performance in multiple C. glutamicum strains (SL4, ATCC 13869, ATCC 13032).
Main Results:
- Identified three constitutive promoters with broad applicability: PcysK, PgapA, and PfumC.
- PcysK emerged as the strongest promoter across all tested strains.
- PcysK showed significantly higher strength compared to the inducible Ptac promoter, particularly when using LacZ reporter.
Conclusions:
- PcysK, PgapA, and PfumC are effective constitutive promoters for C. glutamicum.
- PcysK is a novel, highly efficient promoter suitable for metabolic engineering.
- These promoters can serve as valuable biobricks for enhancing synthesis pathways in industrial C. glutamicum strains.
Keywords:
Corynebacterium glutamicumpromoterred fluorescent protein reporter systemtime-series transcriptomeMore Related Videos
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