Murine Models of Intraperitoneal Perfusion for Disseminated Colorectal Cancer

Area of Science:

• Oncology
• Surgical Techniques
• Preclinical Models

Background:

• Hyperthermic intraperitoneal chemotherapy (HIPEC) preclinical models are crucial for evaluating new cancer therapies.
• This study details the technical execution of closed and open abdominal perfusion techniques.
• Focus is on a mouse model of colorectal cancer peritoneal carcinomatosis.

Purpose of the Study:

• To describe and compare closed versus open "coliseum" abdominal perfusion techniques in a murine model.
• To establish a reliable preclinical model for HIPEC research.
• To investigate the feasibility of these perfusion methods for delivering novel therapeutic agents.

Main Methods:

• BALB/c mice with disseminated colorectal cancer (CT26-luciferin) underwent 30-minute perfusions.
• Two techniques were compared: closed perfusion and open "coliseum" perfusion.
• Disease monitoring utilized in vivo imaging; perfusion circuits were detailed.

Main Results:

• Closed perfusion (12 mice) experienced complications like perfusate leakage and organ suction.
• Coliseum perfusion (9 mice), with tumor debulking, resulted in survival with limited intraoperative issues.
• Tumor debulking involved bipolar cauterization of peritoneal tumors.

Conclusions:

• The coliseum perfusion technique demonstrated fewer intraoperative complications than the closed method.
• These described methods serve as a guideline for developing future murine perfusion models.
• The models are suitable for investigating chemotherapy delivery and other tumor-sensitization agents.