Related Experiment Video
Updated: Feb 1, 2026
12:55
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
19.2K
Phage Display Technology for Human Monoclonal Antibodies
Marco Dal Ferro1, Serena Rizzo1, Emanuela Rizzo1
1Department of Life Sciences, University of Trieste, Trieste, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2018
Summary
Phage display technology enables the creation of large antibody libraries for rapid screening. This method allows for the isolation and engineering of functional antibody fragments with high affinity and specificity.
Area of Science:
- Biotechnology
- Immunology
- Molecular Biology
Background:
- In vitro technologies have advanced antibody discovery over the past 20 years.
- Filamentous phage display is a highly successful method for generating and selecting antibody libraries.
Purpose of the Study:
- To describe protocols for constructing antibody libraries using phage display.
- To outline methods for selecting antibody binders with specificities of interest.
Main Methods:
- Displaying functional antibody fragments on filamentous phages by fusing antibody variable regions to the phage minor coat protein pIII.
- Utilizing large library generation combined with rapid selection and screening procedures.
- Employing cycles of selection on the antigen of interest to isolate desired antibodies.
Main Results:
- Antibodies with affinities comparable to hybridoma technology can be isolated.
- Antibody genes can be recovered simultaneously with selection.
- Engineered antibodies can achieve affinities and effector functions unobtainable through traditional immunization.
Conclusions:
- Phage display technology provides a powerful platform for antibody library construction and selection.
- This method facilitates the isolation of high-affinity antibodies and allows for further engineering of specificity and effector functions.
Related Concept Videos
01:10Antibody Structure
65.5K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.5K
01:31Hybridoma Technology
17.7K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
17.7K
01:30Health Information Technology and Healthcare Information System
3.4K
Health Information Technology (HIT)
Health Information Technology, commonly called HIT, integrates advanced information systems and technology in healthcare settings. Its primary functions include:
Health Information Technology, commonly called HIT, integrates advanced information systems and technology in healthcare settings. Its primary functions include:
3.4K
01:26Antibody Actions
2.7K
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
2.7K
01:25Antibody Structure and Classes
9.1K
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
9.1K
01:24Biodiversity and Human Values
17.1K
Human civilization relies on biodiversity in many ways. Sudden changes in species biodiversity result in environmental changes that can modify weather patterns and therefore human civilizations.
17.1K