Fluorogenic probes for super-resolution microscopy.

Area of Science:

• Biochemistry
• Microscopy
• Molecular Imaging

Background:

• Fluorogenic probes significantly improve signal-to-noise ratios by minimizing non-specific background signals.
• Super-resolution microscopy (SRM) benefits from enhanced resolution, yet fluorogenic probes are underutilized in this field.
• Existing reviews focus on SRM techniques or fluorophores, lacking a dedicated summary of fluorogenic probes for SRM.

Purpose of the Study:

• To provide a comprehensive review of fluorogenic probes specifically for super-resolution microscopy.
• To highlight the importance and application of fluorogenic probes in advancing super-resolution imaging.
• To fill the existing gap in literature regarding fluorogenic probes in SRM.

Main Methods:

• Literature review of fluorogenic probes and their application in super-resolution microscopy.
• Synthesis of information on probe design, mechanisms, and performance in SRM.
• Analysis of current trends and future prospects of fluorogenic probes in cellular imaging.

Main Results:

• Fluorogenic probes offer a powerful strategy to achieve high signal-to-noise ratios in super-resolution imaging.
• A wide array of fluorogenic probes have been developed and applied to visualize cellular structures with enhanced resolution.
• The review consolidates information on various fluorogenic probes suitable for different super-resolution techniques.

Conclusions:

• Fluorogenic probes are crucial for overcoming background noise and improving resolution in super-resolution microscopy.
• This review serves as an up-to-date resource for researchers utilizing or developing fluorogenic probes for advanced cellular imaging.
• Further exploration of fluorogenic probes will continue to drive innovation in super-resolution microscopy.