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Updated: Feb 1, 2026
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Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
Published on: September 30, 2018
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Single-Cell Transcriptome Profiling of Mouse and hESC-Derived Pancreatic Progenitors
Nicole A J Krentz1, Michelle Y Y Lee2, Eric E Xu1
1Diabetes Research Group, BC Children's Hospital Research Institute, Vancouver, BC V5Z 4H4, Canada; Departments of Surgery and Cellular and Physiological Sciences, University of British Columbia, 950 28(th) Avenue West, Vancouver, BC V5Z4H4, Canada.
Stem Cell Reports
|December 13, 2018
Summary
This study maps cell types in developing mouse pancreas and human stem cells to improve diabetes treatment. Understanding endocrine progenitor development aids in generating insulin-producing beta cells from human embryonic stem cells.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Endocrinology
Background:
- Human embryonic stem cells (hESCs) offer a potential source of insulin-producing beta cells for diabetes therapy.
- Improved understanding of beta cell development is crucial for refining hESC differentiation protocols.
Purpose of the Study:
- To characterize the single-cell transcriptomes of mouse and hESC-derived endocrine progenitors.
- To identify key cell-state populations involved in pancreatic endocrine development.
- To provide a resource for enhancing the generation of functional beta-like cells from hESCs.
Main Methods:
- Single-cell RNA sequencing of mouse embryonic pancreas (E15.5 and E18.5) and hESC-derived endocrine progenitors.
- Utilized Neurog3-Cre; Rosa26mT/mG mouse model for lineage tracing.
- Employed a NEUROG3-2A-eGFP hESC reporter line (N5-5) for isolating GFP+ cells.
Main Results:
- Successfully sequenced transcriptomes of 6,905 mouse and 6,626 mouse embryonic day 15.5/18.5 pancreatic cells, and 4,462 hESC-derived GFP+ cells.
- Differential gene expression analysis identified distinct progenitor, endocrine, and novel cell-state populations.
- Established a comprehensive single-cell atlas of pancreatic endocrine progenitor development.
Conclusions:
- The study provides a valuable single-cell transcriptomic resource for pancreatic development.
- Findings will aid in optimizing protocols for generating insulin-producing beta cells from hESCs.
- This research advances the potential for cell-based therapies for diabetes.
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