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Updated: Feb 1, 2026

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes
Published on: August 16, 2018
Ultrathin two-dimensional covalent organic framework nanoprobe for interference-resistant two-photon fluorescence
Peng Wang1, Fang Zhou1, Cheng Zhang1
1Molecular Science and Biomedicine Laboratory , State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry and Chemical Engineering , College of Life Sciences , Collaborative Innovation Center for Chemistry and Molecular Medicine , Hunan University , Changsha , Hunan 410082 , China .
We developed a new covalent organic framework (COF) nanoprobe, TpASH-NPHS, for accurate bioimaging. This probe overcomes interference from biological molecules, enabling precise detection of hydrogen sulfide (H2S) in complex environments.
Area of Science:
- Biomedical Engineering
- Materials Science
- Analytical Chemistry
Background:
- Living organisms present complex environments that challenge the selectivity of traditional small-molecule fluorescent probes for bioimaging.
- Covalent organic frameworks (COFs) offer a unique solution due to their structured pores, enabling interference filtering and accurate biosensing.
Purpose of the Study:
- To develop an interference-resistant hybrid probe by combining COFs and small-molecule probes for enhanced bioimaging.
- To create a two-photon fluorescent COF nanoprobe (TpASH-NPHS) for targeting and detecting hydrogen sulfide (H2S).
Main Methods:
- Fabrication of a novel two-photon fluorescent COF nanoprobe, TpASH-NPHS.
- Evaluation of TpASH-NPHS for targeting hydrogen sulfide (H2S) as a model analyte.
- Comparison of TpASH-NPHS performance against traditional small-molecule probes in complex biological environments.
Main Results:
- TpASH-NPHS demonstrated limited cytotoxicity, excellent photostability, and long-term bioimaging capability.
- The COF-based probe achieved accurate H2S detection, unaffected by interference from intracellular enzymes, unlike small-molecule probes.
- Successful monitoring of endogenous H2S levels in a mouse model of cirrhosis was achieved.
Conclusions:
- The developed COF-based hybrid probe offers an effective strategy for interference-resistant biosensing.
- TpASH-NPHS provides a robust platform for accurate detection and imaging of biologically relevant analytes like H2S.
- This approach holds significant potential for advancing in vivo bioimaging and disease diagnostics.
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