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Updated: Feb 1, 2026

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
Published on: December 22, 2017
Isolation and culture of human primary keratinocytes-a methods review
Aneta Ścieżyńska1,2, Aleksandra Nogowska1, Maria Sikorska3,4
1Department of Histology and Embryology, Medical University of Warsaw, Warsaw, Poland.
Abstract:
Keratinocyte culture is a necessary and widely used tool in a variety of experimental dermatological and biomedical studies. Literature search has revealed a variety of different protocols of human primary keratinocyte isolation and culture. Therefore, the aim of this paper was to review and summarize current trends in human primary keratinocyte culture techniques. We present data on the most popular and effective methods of human keratinocyte isolation and cultivation obtained from screening of 945 papers published during the last 10 years.
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