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Genes coding for RNA polymerase beta subunit in bacteria. Structure/function analysis.

N A Lisitsyn1, G S Monastyrskaya, E D Sverdlov

  • 1Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.

European Journal of Biochemistry
|November 1, 1988
PubMed
Summary
This summary is machine-generated.

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The rpoB gene sequence from Salmonella typhimurium was determined and compared to other bacterial sequences. This analysis revealed conserved regions, offering insights into bacterial RNA polymerase structure and function.

Area of Science:

  • Molecular Biology
  • Genomics
  • Bacterial Genetics

Background:

  • The rpoB gene encodes the beta subunit of bacterial RNA polymerase, a crucial enzyme for transcription.
  • Understanding the structure and function of RNA polymerase is vital for deciphering gene regulation in bacteria.

Purpose of the Study:

  • To determine the nucleotide sequence of the rpoB gene in Salmonella typhimurium.
  • To compare this sequence with known rpoB sequences from other bacterial species.
  • To identify conserved regions within the rpoB gene and infer functional and structural insights.

Main Methods:

  • DNA sequencing to determine the rpoB gene nucleotide sequence.
  • Bioinformatic analysis for sequence comparison and identification of conserved regions.

Related Experiment Videos

  • Comparative genomics approaches.

    • Main Results:

    • The complete nucleotide sequence of the Salmonella typhimurium rpoB gene was elucidated.
    • Identification of highly conserved nucleotide regions across different bacterial species.
    • Comparative analysis provided data on the distribution of functional domains within the RNA polymerase beta subunit.

    Conclusions:

    • Conserved regions in the rpoB gene correlate with essential functional domains of the RNA polymerase beta subunit.
    • The findings contribute to understanding the three-dimensional structure and evolutionary conservation of bacterial RNA polymerase.