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Target-driven switch-on fluorescence aptasensor for trace aflatoxin B1 determination based on highly fluorescent
Xiaoting Lu1, Chengquan Wang2, Jing Qian1
1School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China.
Analytica Chimica Acta
|December 21, 2018
Summary
A new fluorescence aptasensor uses cadmium zinc telluride quantum dots and gold nanoparticles to detect trace aflatoxin B1 (AFB1). This sensitive method offers a simple, separation-free approach for AFB1 determination in food samples.
Area of Science:
- Analytical Chemistry
- Nanotechnology
- Biochemistry
Background:
- Aflatoxin B1 (AFB1) poses significant health risks due to its toxicity and carcinogenicity.
- Sensitive and reliable detection methods for trace levels of AFB1 are crucial for food safety.
Purpose of the Study:
- To develop a novel, sensitive, and easy-to-use fluorescence aptasensor for the determination of trace aflatoxin B1.
- To utilize the fluorescence resonance energy transfer (FRET) between novel quantum dots and gold nanoparticles for AFB1 detection.
Main Methods:
- Preparation of 3-mercaptopropionic acid (MPA)-capped ternary cadmium zinc telluride (CdZnTe) quantum dots (QDs) via a hydrothermal route.
- Fabrication of a FRET-based aptasensor using CdZnTe QDs as donors and gold nanoparticles (AuNPs) as acceptors, functionalized with AFB1 aptamers and complementary DNA.
- Detection mechanism based on the disruption of FRET upon AFB1 binding, leading to fluorescence recovery of CdZnTe QDs.
Main Results:
- The developed CdZnTe QDs exhibited enhanced fluorescence intensity compared to binary CdTe QDs.
- The aptasensor demonstrated a switch-on fluorescence response to AFB1.
- A broad detection range of 50 pg/mL to 100 ng/mL was achieved with good sensitivity.
- Successful application of the aptasensor in spiked peanut samples.
Conclusions:
- A novel and effective fluorescence aptasensor for trace AFB1 detection was successfully developed.
- The CdZnTe QDs-AuNPs FRET pair offers a promising platform for aptasensor fabrication.
- The separation-free and user-friendly nature of the aptasensor facilitates practical applications in food safety monitoring.
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