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Related Concept Videos

Isothermal Processes01:21

Isothermal Processes

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A thermodynamic process that occurs at constant temperature is called an isothermal process. Heat slowly flows into the system or out of the system to maintain thermal equilibrium. Processes involving phase changes like water evaporation into steam or freezing water into ice at a constant temperature are examples of Isothermal Processes.
An ideal gas can also undergo isothermal expansion or compression.
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RACE - Rapid Amplification of cDNA Ends02:35

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Animal organs and organ systems constantly adjust to internal and external changes through a process called homeostasis ("steady state"). Examples of these changes include regulation of the level of glucose or calcium in the blood or internal responses to external temperatures. Homeostasis requires  maintaining an internal dynamic equilibrium:
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Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
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Loop-mediated isothermal amplification-based microfluidic chip for pathogen detection.

Yuting Shang1, Jiadi Sun1, Yongli Ye1

  • 1State Key Laboratory of Food Science and Technology School of Food Science National Engineering Research Center for Functional Foods, Synergetic Innovation Center of Food Safety, Joint International Research Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China.

Critical Reviews in Food Science and Nutrition
|December 21, 2018
PubMed
Summary
This summary is machine-generated.

Rapid detection of foodborne pathogens is crucial due to increasing contamination risks. Loop-mediated isothermal amplification (LAMP) on microfluidic chips offers a sensitive, point-of-care solution for enhanced food safety inspections.

Keywords:
Loop-mediated isothermal amplificationmicrofluidic chippathogens

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Area of Science:

  • Food safety and microbiology
  • Biotechnology and molecular diagnostics
  • Microfluidics and lab-on-a-chip technologies

Background:

  • Rising global food production increases contamination risks and foodborne illnesses.
  • Traditional bacterial culturing methods are insufficient for modern food safety demands.
  • Emerging pathogenic microorganisms necessitate advanced detection strategies.

Purpose of the Study:

  • To review recent advancements in loop-mediated isothermal amplification (LAMP) for pathogen detection.
  • To highlight the integration of LAMP assays on microfluidic chips.
  • To provide guidelines for molecular diagnosis and genetic analysis in resource-limited settings.

Main Methods:

  • Overview of loop-mediated isothermal amplification (LAMP) techniques.
  • Analysis of microfluidic chip integration for LAMP assays.
  • Discussion of real-time and endpoint detection mechanisms.

Main Results:

  • LAMP technology enables rapid and sensitive nucleic acid amplification under isothermal conditions.
  • Microfluidic integration enhances screening efficiency and point-of-care applicability.
  • LAMP-based microfluidic chips offer a viable alternative to conventional methods.

Conclusions:

  • LAMP on microfluidic chips is a promising technology for rapid pathogen detection.
  • This integrated approach supports food safety inspections and quality assurance.
  • Further development can enhance molecular diagnostics and genetic analysis capabilities.