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Updated: Jan 31, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda
Published on: October 14, 2011
PhageFISH for Monitoring Phage Infections at Single Cell Level
Jimena Barrero-Canosa1, Cristina Moraru2
1Department of Environmental Technology., Technische Universität Berlin, Berlin, Germany.
Abstract:
PhageFISH uses the power of fluorescence in situ hybridization to monitor intracellular phage infections at single cell level. It combines host cell identification via rRNA probes and phage identification via phage-specific gene probes, allowing for the quantification of the infected cell fraction and the discrimination between infection stages. This book chapter covers all aspects of the procedure, from phage probe design and synthesis, to the phageFISH protocol itself, to microscopy and image analysis.
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