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Updated: Jan 31, 2026

Synthesis of an Intein-mediated Artificial Protein Hydrogel
Published on: January 27, 2014
Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform
Samuel Lim1, Gi Ahn Jung, Riya J Muckom
1Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA. dsc@berkeley.edu.
Abstract:
We demonstrate the synthesis of protein-polymer hybrid hydrogel that can be used as a platform for immobilizing functional proteins. Orthogonal chemistry was employed for cross-linking the hybrid network and conjugating proteins to the gel backbone, allowing for the convenient, one-pot formation of a functionalized hydrogel. The resulting hydrogel had tunable mechanical properties, was stable in solution, and biocompatible.
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