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Updated: Jan 31, 2026

Probiotic Studies in Neonatal Mice Using Gavage
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Electrospun probiotics: an alternative for encapsulation.

Z Akbar1, T Zahoor1, N Huma1

  • 1NIFSAT, University of Agriculture, Faisalabad, Pakistan.

Journal of Biological Regulators and Homeostatic Agents
|December 22, 2018
PubMed
Summary
This summary is machine-generated.

Electrospinning poly(vinyl alcohol) fibers successfully encapsulated Bifidobacterium animalis subsp. Lactis Bb12 and a probiotic combination, maintaining viability for one week at room and refrigeration temperatures. This method offers a promising approach for prolonged probiotic delivery.

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Microbiology

Background:

  • Electrospinning is a versatile technique for fabricating nonwoven nanofibers with biomedical applications.
  • Poly(vinyl alcohol) (PVA) is a GRAS material suitable for electrospinning due to its oxygen barrier and water solubility properties, facilitating probiotic recovery.
  • This study optimizes electrospinning parameters for probiotic encapsulation and assesses their viability post-fabrication.

Discussion:

  • Probiotic viability was maintained for one week in PVA nanofibers at room temperature (25°C) and refrigeration (4°C), but not at -20°C.
  • The 9% PVA concentration yielded homogenous, uniform, bead-free nanofibers with excellent probiotic encapsulation efficiency.
  • Probiotic 1 (Bifidobacterium animalis subsp. Lactis Bb12) and Probiotic 2 (combination) showed high encapsulation efficiencies (84-93%) and maintained bacterial counts in the 10^8 CFU/mL range.

Key Insights:

  • Electrospun PVA nanofibers effectively preserve probiotic viability for extended periods.
  • Optimized electrospinning conditions (9% PVA) result in high-quality nanofibers suitable for probiotic delivery.
  • Probiotics encapsulated in PVA nanofibers demonstrate significant stability at ambient and refrigerated conditions.

Outlook:

  • This technology holds potential for developing novel delivery systems for probiotics and other bioactive agents.
  • Further research can explore the long-term stability and in vivo efficacy of these probiotic-loaded nanofibers.
  • The GRAS status of PVA and ease of probiotic recovery support potential food and pharmaceutical applications.