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Related Experiment Video

Updated: Jan 31, 2026

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
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ALPHABETA: a dedicated open-source tool for calculating TEM stage tilt angles.

N Cautaerts1,2, R Delville1, D Schryvers2

  • 1Fuel Materials Group, Institute for Nuclear Materials Science, Mol, Belgium.

Journal of Microscopy
|December 25, 2018
PubMed
Summary

ALPHABETA software simplifies transmission electron microscopy (TEM) by calculating precise tilt angles for sample orientation. This open-source tool reduces experimental time and enhances accuracy for various TEM applications.

Keywords:
CrystallographyTEMdiffractionsoftwaretiltingtool

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Area of Science:

  • Materials Science
  • Physics
  • Chemistry

Background:

  • Transmission Electron Microscopy (TEM) requires precise sample orientation for optimal imaging.
  • Manual adjustment of tilt angles on TEM stages is time-consuming and requires expertise.
  • Accurate orientation is crucial for analyzing material structures and properties.

Purpose of the Study:

  • To introduce ALPHABETA, an open-source software for calculating TEM sample tilt angles.
  • To provide an intuitive tool for calibrating sample orientation and achieving specific crystallographic conditions.
  • To reduce the time and complexity associated with TEM tilting experiments.

Main Methods:

  • Development of a mathematical framework for tilt angle calculation.
  • Implementation of an open-source software with a user-friendly interface.
  • Evaluation of the software's accuracy in reaching target zone axes and experimental conditions.

Main Results:

  • ALPHABETA accurately calculates tilt angles for various TEM operations, including zone axis targeting and 2-beam conditions.
  • The software visualizes sample orientation and reachable tilt domains using stereographic projection.
  • Case studies demonstrate the software's effectiveness in reducing tilting time and enabling new applications like dark-field tilt series.

Conclusions:

  • ALPHABETA significantly streamlines TEM tilting procedures, making complex orientations more accessible.
  • The software enhances experimental efficiency and accuracy for researchers using TEM.
  • ALPHABETA supports diverse TEM applications beyond standard zone axis tilting.