Universal method for direct bioconjugation of electrode surfaces by fast enzymatic polymerization

Area of Science:

• Biomaterials Science
• Electrochemistry
• Biosensing

Background:

• Enzyme-catalyzed polymerization offers a pathway to create functional biomaterials.
• Covalent immobilization of biomolecules is crucial for developing stable biosensors.
• Tannic acid (TA) is a natural polyphenol with potential for material functionalization.

Purpose of the Study:

• To develop a versatile platform for covalent immobilization of biomolecules using HRP-catalyzed polymerization of TA.
• To explore the application of poly(Tannic acid) (p(TA)) for capturing cancer cells.
• To investigate the use of p(TA)-modified surfaces for electrochemical sensing of biomolecules.

Main Methods:

• Horseradish peroxidase (HRP)-catalyzed polymerization of tannic acid (TA) to form poly(Tannic acid) (p(TA)).
• Electrochemical oxidation of p(TA) for surface modification and covalent immobilization of biomolecules.
• Utilizing folic acid (FA) as a linker for capturing cancer cells.
• Simultaneous electrochemical determination of ascorbic acid (AA), dopamine (DA), and uric acid (UA) on p(TA)-modified electrodes.

Main Results:

• Successful HRP-catalyzed polymerization of TA and formation of a versatile p(TA) platform.
• Demonstrated covalent immobilization of biomolecules onto various electrode surfaces via p(TA).
• Achieved capture of cancer cells using FA-functionalized p(TA) surfaces.
• Exhibited enhanced electrocatalytic activity for simultaneous detection of AA, DA, and UA.

Conclusions:

• HRP-catalyzed TA polymerization provides a simple and effective method for creating multifunctional platforms.
• The p(TA)-mediated surface modification enables covalent attachment of biomolecules and sensitive electrochemical sensing.
• This approach offers a new framework for developing advanced biosensing devices and targeted cell capture systems.