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Updated: Jan 31, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Screening method for simultaneous detection of elaidic and vaccenic trans fatty acid isomers by capillary zone
Tatiane Lima Amorim1, Lucas Mattos Duarte1, Hélio F Dos Santos2
1Grupo de Química Analítica e Quimiometria - GQAQ, Department of Chemistry, Institute of Exact Sciences, Federal University of Juiz de Fora, 36036-330, Juiz de Fora, MG, Brazil.
Abstract:
A novel screening method for the simultaneous detection of elaidic and vaccenic trans fatty acid isomers by CZE-UV was proposed and applied to brazilian spreadable cheese analysis. Optimized background electrolyte was composed by 10 mmol L-1 heptakis (2, 3, 6-tri-o-methyl)-beta cyclodextrin, 24 mmol L-1 sodium tetraborate buffer solution, 45% methanol and 15% acetonitrile. Sample preparation comprised only a saponification reaction and dilution of sample solution before analysis. The method proved to be simple, environmental friendly and sensitive (LOD calculated for elaidic and vaccenic acid were 0.0163 mmol L-1 and 0.0169 mmol L-1, respectively) and may be used to detect adulteration in dairy products, where the addition of hydrogenated vegetable fat to food is prohibited.
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