Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Practical diagnostic testing for human immunodeficiency virus.

J B Jackson1, H H Balfour

  • 1Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455.

Clinical Microbiology Reviews
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Post-TB lung function, quality of life, and radiographic findings in children.

IJTLD open·2025
Same author

Conducting interslice stimulation for concurrent TMS-fMRI.

Journal of neuroscience methods·2025
Same author

Domain general frontoparietal regions show modality-dependent coding of auditory and visual rules.

bioRxiv : the preprint server for biology·2024
Same author

Predictors of mortality in Ugandan children with TB, 2016-2021.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2023
Same author

Screening for Epstein-Barr virus (EBV) infection status in university freshmen: acceptability of a gingival swab method.

Epidemiology and infection·2019
Same author

The impact of recipient BKV shedding before transplant on BKV viruria, DNAemia, and nephropathy post-transplant: A prospective study.

Pediatric transplantation·2017
Same journal

Diagnostic challenges in re-emerging rickettsioses: why current tools fall short.

Clinical microbiology reviews·2026
Same journal

Current progress in developing mRNA-lipid nanoparticle vaccines against human bacterial pathogens.

Clinical microbiology reviews·2026
Same journal

The gut microbiome and colorectal cancer.

Clinical microbiology reviews·2026
Same journal

Influence of fungi on epithelial homeostasis and role in inflammatory diseases.

Clinical microbiology reviews·2026
Same journal

Coxsackievirus A6 on the rise: epidemiology, pathogenicity, evolutionary dynamics, and antiviral strategy.

Clinical microbiology reviews·2026
Same journal

Intravesical therapies for catheter-associated urinary tract infections: current approaches and future directions.

Clinical microbiology reviews·2026
See all related articles

Numerous diagnostic tests for human immunodeficiency virus (HIV) are available, detecting antibodies, antigens, and genetic material. Key assays like ELISA and Western blot offer high sensitivity and specificity for HIV detection.

Area of Science:

  • Virology
  • Immunology
  • Diagnostic Medicine

Background:

  • Human immunodeficiency virus (HIV) identified in 1983 as the cause of AIDS.
  • Significant advancements in diagnostic testing have emerged since HIV discovery.

Purpose of the Study:

  • To review available diagnostic tests for HIV.
  • To assess the sensitivity, specificity, and adaptability of various HIV assays.

Main Methods:

  • Review of currently available HIV diagnostic assays.
  • Categorization of tests based on what they detect (antibody, antigen, nucleic acids, reverse transcriptase).
  • Evaluation of Enzyme-linked immunosorbent assays (ELISA), Western blot, radioimmunoprecipitation assays, indirect immunofluorescence assays, reverse transcriptase assays, and molecular hybridization techniques.

Related Experiment Videos

Main Results:

  • ELISA, Western blot, and indirect immunofluorescence assays show high sensitivity and specificity for HIV antibody detection.
  • HIV antigen detection via ELISA is adaptable for most labs and nearing commercial availability.
  • Other assays, though more complex, serve as valuable confirmatory tests for reference laboratories.

Conclusions:

  • A wide array of HIV diagnostic tools are available, catering to different laboratory settings.
  • Standardized and sensitive assays like ELISA and Western blot are suitable for routine HIV screening.
  • Proper biosafety measures are crucial for minimizing biohazards during HIV testing.