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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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Lagging Strand Synthesis01:59

Lagging Strand Synthesis

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During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
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Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

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The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
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Assembly of Complex Microtubule Structures01:32

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Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
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DNA Helicases00:55

DNA Helicases

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DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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The DNA Replication Fork01:02

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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Updated: Jan 31, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
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Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

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Triangulated Wireframe Structures Assembled Using Single-Stranded DNA Tiles.

Michael Matthies, Nayan P Agarwal, Erik Poppleton1

  • 1Center for Molecular Design and Biomimetics, The Biodesign Institute , Arizona State University , 1001 South McAllister Avenue , Tempe , Arizona 85281 , United States.

ACS Nano
|January 10, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed new DNA structures using a single-stranded tile method. These DNA nanostructures are stable in biological conditions and allow for precise control over shape and size.

Keywords:
molecular dynamics simulationssingle-stranded tilesstructural DNA nanotechnologytriangulated wireframe structures

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Area of Science:

  • Structural DNA nanotechnology
  • DNA self-assembly

Background:

  • Traditional DNA structures face limitations in stability and material efficiency.
  • Triangulated wireframe DNA structures offer improved stability and reduced material usage.
  • Anisotropic and finite structures are desirable for advanced applications.

Purpose of the Study:

  • Expand the design space of the DNA single-stranded tile method.
  • Develop anisotropic, finite, triangulated wireframe DNA structures.
  • Create one-dimensional crystalline DNA assemblies.

Main Methods:

  • Utilized the DNA single-stranded tile method.
  • Employed six-arm junctions with single double helix edges.
  • Incorporated single-stranded spacers (2-4 nucleotides) in junctions.
  • Performed coarse-grained molecular dynamics simulations with the oxDNA model.

Main Results:

  • Successfully designed and simulated a range of anisotropic, finite, triangulated wireframe structures.
  • Demonstrated the assembly of one-dimensional crystalline DNA assemblies.
  • Identified the crucial role of spacers in preventing helix stacking.
  • Confirmed facilitation of planar geometry assembly.

Conclusions:

  • The DNA single-stranded tile method is versatile for creating complex DNA nanostructures.
  • Spacers are essential for reliable folding and achieving desired geometries.
  • These DNA nanostructures exhibit enhanced stability in physiological conditions.