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Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
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Visualization and Cytotoxicity of Fluorescence-Labeled Dimeric Magnetite-Gold Nanoparticles Conjugated with

S K Pirutin1,2, M V Efremova3,4, A I Yusipovich3

  • 1M. V. Lomonosov Moscow State University, Moscow, Russia. pirutin@yandex.ru.

Bulletin of Experimental Biology and Medicine
|January 11, 2019
PubMed

Insights

Magnetite-gold nanoparticles conjugated with prostate-specific membrane antigen can enter mouse macrophages. While uptake was limited, nanoparticles were found in the cytoplasm, with some cells showing membrane damage, impacting oncology applications.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Oncology

Background:

  • Nanoparticles are increasingly used in oncology for diagnostics and therapeutics.
  • Targeted delivery of nanoparticles to specific cells is crucial for efficacy.
  • Understanding nanoparticle-cell interactions, including uptake and potential toxicity, is essential.

Purpose of the Study:

  • To investigate the cellular uptake of magnetite-gold nanoparticles conjugated with prostate-specific membrane antigen in mouse macrophages.
  • To assess the intracellular localization and potential cytotoxic effects of these nanoparticles.

Main Methods:

  • Incubation of mouse macrophages with magnetite-gold nanoparticles conjugated with prostate-specific membrane antigen.
  • Quantification of nanoparticle uptake using microscopy.
  • Assessment of macrophage membrane integrity.

Main Results:

  • Magnetite-gold nanoparticles conjugated with prostate-specific membrane antigen demonstrated limited uptake (13%) in mouse macrophages after 3-hour incubation at 15 mg/L and 37°C.
  • Nanoparticles were predominantly found within the cytoplasm (approx. 90% of positive cells).
  • Cell membrane damage was observed in 25% of macrophages under these conditions.

Conclusions:

  • The study shows that targeted nanoparticles can penetrate macrophages, but with limited efficiency.
  • Intracellular localization and potential for membrane damage require consideration for safe and effective nanomedicine development.
  • These findings are critical for the future design of nanomaterials in cancer diagnostics and therapy.
Keywords:
cell membranesmacrophagesmagnetite-gold nanoparticlesprostate-specific membrane antigen

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