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Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
Published on: September 26, 2013
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Isolation and Characterisation of Human Adipose-Derived Stem Cells
Anna Wilson1, Melisse Chee1, Peter Butler1
1Research Department of Surgical Biotechnology, UCL Division of Surgery and Interventional Science, University College London, Royal Free Hospital, London, UK.
Methods in Molecular Biology (Clifton, N.J.)
|January 17, 2019
Summary
Adipose-derived stem cells (ASCs) show promise as immunomodulatory agents. This study introduces fluorescence-activated cell sorting (FACS) for enhanced purification of ASCs for clinical applications.
Area of Science:
- Stem cell biology
- Immunology
- Biotechnology
Background:
- Adipose-derived stem cells (ASCs) possess immunomodulatory properties.
- Current ASC isolation methods involve enzymatic digestion, centrifugation, and filtration.
- There is a need for improved purification techniques for clinical applications.
Purpose of the Study:
- To optimize the isolation and purification of adipose-derived stem cells (ASCs).
- To evaluate the utility of fluorescence-activated cell sorting (FACS) in ASC purification.
- To provide a method for obtaining clinical-grade ASCs.
Main Methods:
- Isolation of ASCs from human lipoaspirate via enzymatic digestion.
- Purification of putative ASCs using fluorescence-activated cell sorting (FACS).
- Standard cell culture and characterization techniques.
Main Results:
- Successful isolation of ASCs from lipoaspirate.
- FACS enabled enhanced purification of ASCs compared to standard methods.
- Purified ASCs demonstrated potential for downstream applications.
Conclusions:
- FACS is an effective method for purifying ASCs.
- This technique can yield high-purity ASCs for clinical use.
- Optimized ASC purification supports regenerative medicine and immunotherapy.
Keywords:
ASCsAdipose stem cellsAdipose-derived stem cellsCharacterizationCollagenaseImmunomodulationImmunosuppressionIsolationLipoaspirateLipotransferStromal vascular fractionMore Related Videos
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