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Sieve-through vertical flow platform for efficient liquid exchange in particle-based assays.
Yi Zhang1, Daniel Yoke San Lee1, Aysha Farwin1
1NanoBio Lab, 31 Biopolis Way, The Nanos, Singapore, 138669, Singapore.
Analytica Chimica Acta
|January 22, 2019
Summary
A novel sieve-through platform minimizes reagent carry-over contamination in particle-based assays. This innovation enhances liquid exchange efficiency for improved protein and cell analysis, enabling high-throughput applications.
Area of Science:
- Biomedical engineering
- Assay development
- Microfluidics
Background:
- Particle-based assays are crucial in biomedicine but suffer from carry-over contamination.
- Residual reagents from liquid exchange compromise assay performance and accuracy.
Purpose of the Study:
- To develop and characterize a sieve-through platform for efficient liquid exchange in particle-based assays.
- To reduce carry-over contamination and improve the performance of particle-based systems.
- To demonstrate the platform's utility in protein and cell analysis and high-throughput applications.
Main Methods:
- Development of a sieve-through platform using a porous membrane and absorbent pad.
- Investigation of factors influencing flow characteristics through the porous membrane.
- Application of the platform for particle-based enzyme-linked immunosorbent assay (ELISA).
- Demonstration of a sieve-array for parallel, high-throughput analysis.
Main Results:
- The sieve-through platform effectively removes waste liquid via capillary action.
- Carry-over contamination was significantly reduced compared to conventional methods.
- Successful implementation of particle-based ELISA for protein and cell detection was achieved.
- A sieve-array format demonstrated potential for high-throughput screening.
Conclusions:
- The sieve-through platform offers a robust solution for efficient liquid exchange in particle-based assays.
- This technology minimizes carry-over contamination, enhancing assay reliability and sensitivity.
- The platform supports diverse applications, including high-throughput analysis, advancing biomedical research.
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