Related Experiment Video
Updated: Jan 30, 2026

Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks MOFs
Published on: January 17, 2020
Evaluation of polysaccharide-based chiral stationary phases in modern SFC-MS/MS for enantioselective bioanalysis
Liuxi Chen1, Brian Dean1, Xiaorong Liang1
1Drug Metabolism & Pharmacokinetics, Genentech Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
Abstract:
Aim: The applicability of polysaccharide-based chiral stationary phases in modern supercritical fluid chromatography (SFC)-MS/MS for chiral bioanalysis was evaluated. Materials & methods: Ten popular polysaccharide-based chiral stationary phases (CSPs) were tested using a set of 23 drugs against three cosolvents. The effect of temperature and backpressure on separation was examined. Results: The recommended order of CSPs for screening was determined. Methanol with 0.1% NH4OH is proven to be the first choice of cosolvent. Temperature of 40°C and backpressure of 10 or 15 MPa are recommended starting conditions. Phospholipid elution profiles on the polysaccharide-based CSPs were reported for the first time under SFC conditions. Conclusion: A simplified screening protocol with straightforward method optimization approaches was generated for SFC chiral assay development in a reasonable time frame with a high success rate.
Related Concept Videos
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Biosynthesis of Polysaccharides
Chirality
Chiral objects exhibit a sense of handedness when they interact with another chiral object. For example, our left foot can only fit in the left shoe and not in the right shoe. Achiral objects — objects that have...
Chirality in Nature
Modern Molecular Taxonomy
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the...

