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Updated: Jan 30, 2026

Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
Colorimetric method for glucose detection with enhanced signal intensity using ZnFe2O4-carbon nanotube-glucose
Chengke Wang1, Jiangyu Li, Rong Tan
1College of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, P. R. China. wangck@ujs.edu.cn.
Abstract:
In this paper, a composite material comprised of ZnFe2O4 nanomaterial, carbon nanotubes (CNT) and glucose oxidase (GOD) was synthesized and used for glucose detection. ZnFe2O4-CNT was formed by a one-step solvothermal approach using acid-treated CNT as precursor, then GOD was linked to it by coupling reaction between -NH2 and -COOH. After addition of glucose, which is oxidized by GOD, the intermediate product (H2O2) further oxidizes the 3,3',5,5'-tetramethylbenzidine (TMB) substrate and forms a blue product. This process was accelerated in the presence of peroxidase-mimic ZnFe2O4 nanomaterial and the detected signal intensity was correspondingly enhanced. The linear detection range of glucose was 0.8 to 250 μM, with a limit of detection of 0.58 μM. This may originate from (1) the limited diffusion of intermediate species, which resulted in enhanced local concentrations of reaction compounds; (2) enhanced electron transmission among CNT, GOD and ZnFe2O4; (3) the synergistic enhancement of catalytic activity of ZnFe2O4 compared with other metal oxides; (4) the high loading capacity of ZnFe2O4-CNT for GOD molecules, because of its high surface-to-volume ratio. Meanwhile, this method has reasonable selectivity, stability and reusability and can be used for real serum detection, which may be useful for the development of sensitive biosensors.
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