[SUBSPECIES DIFFERENTIATION OF YERSINIA PESTIS STRAINS BY PCR WITH HYBRIDIZATION-FLUORESCENT DETECTION]
Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
|January 30, 2019
Summary
This study introduces a new PCR method for real-time detection to differentiate Yersinia pestis (Y. pestis) subspecies. This rapid technique effectively distinguishes various Y. pestis strains from different geographical origins.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Yersinia pestis (Y. pestis) is the causative agent of plague.
- Subspecies differentiation is crucial for epidemiological surveillance and understanding plague reservoirs.
- Existing methods may lack the speed and specificity required for rapid identification.
Purpose of the Study:
- To develop a novel method for differentiating Y. pestis strains of various subspecies.
- To utilize Polymerase Chain Reaction (PCR) with hybridization-fluorescent detection in real-time for this purpose.
Main Methods:
- Comparative genomics using Mauve 2.3.1, Mega 5.0, and BLAST algorithms to identify subspecies-specific DNA targets.
- Design and optimization of primers and TAqMan probes for PCR.
- Real-time PCR with hybridization-fluorescent detection.
Main Results:
- Identified DNA targets with marker mutations specific to Caucasus, Altai, Gissar, and Ulegei subspecies.
- Detected strains from the Talass alpine plague reservoir.
- Validated the method's effectiveness on 101 Y. pestis strains from diverse natural foci in Russia and abroad.
Conclusions:
- The developed PCR approach with real-time detection provides a rapid and effective means for Y. pestis subspecies differentiation.
- This method can significantly aid in the epidemiological monitoring of plague.
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