Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoreceptors and Plant Responses to Light02:00

Photoreceptors and Plant Responses to Light

28.4K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
28.4K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
Amines to Amides: Acylation of Amines01:19

Amines to Amides: Acylation of Amines

3.5K
Various carboxylic acid derivatives (such as acid chlorides, esters, and anhydrides) can be used for the acylation of amines to yield amides. The reaction requires two equivalents of amines. The first amine molecule functions as a nucleophile and attacks the carbonyl carbon to produce a tetrahedral intermediate. This is followed by the loss of the leaving group and restoration of the C=O bond.
Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
3.5K
Plant Cell Wall02:43

Plant Cell Wall

60.3K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
60.3K
Plant Hormones01:56

Plant Hormones

27.5K
Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
27.5K
Tonicity in Plants00:53

Tonicity in Plants

59.8K
Tonicity describes the capacity of a cell to lose or gain water. It depends on the quantity of solute that does not penetrate the membrane. Tonicity delimits the magnitude and direction of osmosis and results in three possible scenarios that alter the volume of a cell: hypertonicity, hypotonicity, and isotonicity. Due to differences in structure and physiology, tonicity of plant cells is different from that of animal cells in some scenarios.
59.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TDGF1 Mediates the Oncogenic Effects of the OLMALINC/miR-3614-5p ceRNA Axis in Colon Cancer Through Nodal/Smad2 and Glypican-1/MAPK-AKT Signaling.

Cells·2026
Same author

Optimized protocols for culturing and sectioning mouse intestinal organoids: enhancing efficiency and structural integrity.

MethodsX·2026
Same author

The APT1-NACsa3-GGP1 Module Enhances Salt Tolerance and Regulates Ascorbic Acid Biosynthesis in Medicago.

Plant, cell & environment·2026
Same author

Correction: TSP50 facilitates breast cancer stem cell‑like properties maintenance and epithelial‑mesenchymal transition via PI3K p110α mediated activation of AKT signaling pathway.

Journal of experimental & clinical cancer research : CR·2026
Same author

Ceritinib induces ferroptosis via TRIM21-mediated GLUT1 ubiquitination and AMPK-driven metabolic reprogramming in breast cancer.

iScience·2026
Same author

Discriminating flue-cured tobacco cultivars based on sensory quality and their underlying volatile and chemical profiles.

Frontiers in plant science·2026

Related Experiment Video

Updated: Jan 30, 2026

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
08:31

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Published on: April 10, 2020

10.7K

Dynamic Protein S-Acylation in Plants.

Lihua Zheng1, Peng Liu2, Qianwen Liu3

  • 1State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. caoyezlh@cau.edu.cn.

International Journal of Molecular Sciences
|February 1, 2019
PubMed
Summary

Plant S-acylation, a reversible lipid modification, is crucial for various functions. This review highlights identified S-acylated proteins and enzymes, including the first plant de-S-acylation enzyme, revealing unexplored research areas.

Keywords:
S-acylationlipid modificationprotein S-acyl transferasethioesterase

More Related Videos

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
07:27

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Published on: April 9, 2021

6.0K
Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
10:18

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants

Published on: November 1, 2016

21.8K

Related Experiment Videos

Last Updated: Jan 30, 2026

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
08:31

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Published on: April 10, 2020

10.7K
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
07:27

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Published on: April 9, 2021

6.0K
Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
10:18

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants

Published on: November 1, 2016

21.8K

Area of Science:

  • Plant molecular biology
  • Biochemistry
  • Post-translational modifications

Background:

  • Lipid modification, specifically S-acylation, is a vital post-translational modification in cellular processes.
  • S-acylation is unique due to its reversible nature, making it a significant area of research.
  • Understanding S-acylation in plants is crucial for deciphering plant cell functions.

Purpose of the Study:

  • To review experimentally identified S-acylated proteins in plants.
  • To identify enzymes involved in S-acylation and de-S-acylation in plants.
  • To highlight the functional importance and unexplored aspects of S-acylation in plants.

Main Methods:

  • Literature review of experimentally verified S-acylated proteins in plants.
  • Identification of S-acyl transferases linked to S-acylated proteins.
  • Characterization of the first identified plant protein thioesterase with de-S-acylation activity.

Main Results:

  • Several plant proteins have been experimentally confirmed to undergo S-acylation.
  • Two S-acylated proteins were successfully matched to their corresponding S-acyl transferase.
  • The first plant protein exhibiting de-S-acylation activity, a thioesterase, has been identified.

Conclusions:

  • S-acylation plays a significant role in diverse plant functions.
  • The identification of de-S-acylation enzymes opens new avenues for research.
  • Numerous aspects of S-acylation in plants remain to be explored, offering potential for future discoveries.