Related Experiment Video
Updated: Jan 29, 2026

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
Published on: May 20, 2022
Multispectral tracing in densely labeled mouse brain with nTracer
Douglas H Roossien1, Benjamin V Sadis1, Yan Yan1
1Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA.
Summary:
This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains.
Availability And Implementation:
nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
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