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Confirming QTLs and Finding Additional Loci Responsible for Resistance to Rice Sheath Blight Disease
1Rice Research and Extension Center, University of Arkansas, Stuttgart 72160.
Researchers confirmed a major rice sheath blight resistance QTL, qShB9-2, in field trials. They also identified a new QTL and validated greenhouse methods for marker-assisted selection in rice breeding.
Area of Science:
- Plant Pathology
- Genetics
- Agricultural Science
Background:
- Rice sheath blight (ShB), caused by Rhizoctonia solani AG1-1A, is a major global rice disease.
- Host resistance is the most sustainable management strategy.
- Previous studies mapped ShB quantitative trait loci (QTLs) in a Lemont × Jasmine 85 recombinant inbred line (LJRIL) population using greenhouse methods.
Purpose of the Study:
- To confirm previously identified ShB-resistant QTLs under field conditions.
- To identify new ShB-resistance QTLs.
- To validate greenhouse assay effectiveness for marker-assisted selection (MAS).
Main Methods:
- Evaluated ShB resistance in 216 LJRILs under field conditions in Arkansas, Texas, and Louisiana (2008-2009).
- Utilized microchamber and mist-chamber assays for greenhouse evaluations.
- Employed molecular markers (RM221, RM112, RM215, RM245) for QTL analysis.
Main Results:
- Confirmed the major ShB-QTL qShB9-2 using field data.
- Identified a novel ShB-QTL on chromosome 2 between markers RM221 and RM112.
- Demonstrated that microchamber and mist-chamber assays reliably identified ShB-QTLs in greenhouse settings.
Conclusions:
- Field validation confirmed the major ShB-QTL qShB9-2.
- A new ShB-QTL was identified, expanding the genetic resources for ShB resistance.
- Greenhouse assays are effective for early identification of ShB-QTLs, supporting MAS for rice breeding.
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