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Related Concept Videos

Gene Therapy00:59

Gene Therapy

27.6K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
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Group Therapy01:26

Group Therapy

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Group therapy is a sociocultural approach to psychological treatment, where individuals with shared psychological challenges come together under the guidance of a mental health professional. This therapeutic modality offers unique opportunities for individuals to connect, share, and grow within the context of a supportive group. By fostering mutual understanding and collaboration, group therapy can address a range of psychological concerns effectively, often complementing or surpassing the...
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Gene Flow02:39

Gene Flow

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Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
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Gene Conversion02:08

Gene Conversion

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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
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Gene Families01:57

Gene Families

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Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
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What is Gene Expression?01:42

What is Gene Expression?

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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Updated: Jan 29, 2026

Production and Purification of Baculovirus for Gene Therapy Application
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Production and Purification of Baculovirus for Gene Therapy Application

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Methioninase Gene Therapy.

Robert M Hoffman1,2, Kenji Miki3,4, Waddah Al-Refaie3,4

  • 1AntiCancer, Inc., San Diego, CA, USA. all@anticancer.com.

Methods in Molecular Biology (Clifton, N.J.)
|February 7, 2019
PubMed
Summary
This summary is machine-generated.

Recombinant methioninase (rMETase) gene therapy shows promise for cancer treatment by depleting methionine in cancer cells. Combining gene therapy with selenomethionine enhances its efficacy and induces apoptosis in various cancer models.

Keywords:
AdenovirusBy-stander effectCancerGFPGene cloningGene therapyMethioninaseMethionine dependenceMethylselenolProdrugSelenomethionine

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CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications
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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Cancer cells often exhibit methionine dependence, requiring higher levels of methionine for growth.
  • Recombinant methioninase (rMETase) targets this dependence by degrading methionine.
  • Gene therapy approaches can enhance rMETase delivery and efficacy.

Purpose of the Study:

  • To develop and evaluate gene therapy strategies using recombinant methioninase (rMETase) for cancer treatment.
  • To investigate the synergistic effects of rMETase gene therapy combined with other therapeutic agents.
  • To assess the efficacy of rMETase-based therapies in various cancer cell lines and in vivo models.

Main Methods:

  • Construction of retroviral and adenoviral vectors encoding the P. putida methioninase (METase) gene.
  • Transduction of human cancer cell lines (H460, OVACAR-8, HT1080, A549) and normal fibroblasts with METase vectors.
  • Treatment of cancer cells and tumors with rMETase protein, METase gene therapy, selenomethionine (SeMET), and doxorubicin (DOX).
  • Assessment of intracellular methionine levels, cell viability, apoptosis, tumor growth inhibition, and survival rates.

Main Results:

  • Retroviral and adenoviral METase gene transfer effectively reduced intracellular methionine levels in cancer cells.
  • rMETase gene therapy, particularly with adenoviral vectors, led to high METase expression and synergistic cell killing when combined with rMETase protein.
  • Adenoviral METase-transduced cells combined with SeMET induced apoptosis via mitochondrial damage and oxidative stress, exhibiting a bystander effect.
  • AdMETase/SeMET therapy inhibited tumor growth in rodents and prolonged survival, showing efficacy against drug-resistant lung cancer cells.
  • Combination therapy with Ad-METase/SeMET and doxorubicin delayed tumor growth in a human lung cancer xenograft model.

Conclusions:

  • Methionine restriction (MR) via rMETase gene therapy is a viable strategy for cancer treatment.
  • Combining METase gene therapy with SeMET offers a potent therapeutic approach with a bystander effect.
  • METase gene therapy can overcome resistance to conventional chemotherapy and apoptosis-inducing agents.
  • Further development of METase-based gene therapies holds significant potential for clinical application in oncology.