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Updated: Jan 29, 2026

Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring
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Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring

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A convenient method to generate and maintain poly(A)-encoding DNA sequences required for in vitro transcription of

Patrick Arbuthnot1, Abdullah Ely1, Kristie Bloom1

  • 1Wits/SAMRC Antiviral Gene Therapy Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Private Bag 3, WITS 2050, Johannesburg, South Africa.

Biotechniques
|February 8, 2019
PubMed

Keywords:
transcriptionmRNAplasmidpoly(A)type IIS restriction enzymes

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