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Plate Count Accuracy: Analysts and Automatic Colony Counter Versus a True Count 1
John T Fruin1, Warren S Clark1
1Letterman Army Institute of Research, Department of Nutrition, Food Hygiene Division, Presidio of San Francisco, California 94129.
Journal of Food Protection
|February 9, 2019
Summary
Automatic colony counters (ACC) provide accurate dairy product colony counts comparable to manual methods when plates are properly screened. Further research is recommended for potential inclusion in standard dairy testing guidelines.
Area of Science:
- Microbiology
- Food Science
- Analytical Chemistry
Background:
- Accurate microbial enumeration is crucial for dairy product quality control.
- Manual colony counting can be labor-intensive and prone to variability.
Purpose of the Study:
- To compare the accuracy of automatic colony counter (ACC) colony counts with manual analyst counts and photographic true counts.
- To identify factors affecting ACC performance in dairy product analysis.
Main Methods:
- Colony counts from manual analysts and an ACC were compared against counts derived from photographic standards.
- Factors contributing to counting discrepancies with the ACC were systematically investigated.
Main Results:
- ACC counts demonstrated accuracy comparable to manual counts when plates were appropriately screened.
- Specific factors causing counting difficulties with the ACC were identified and analyzed.
Conclusions:
- Automatic colony counters can achieve accuracy equivalent to manual methods for dairy product microbial analysis.
- Further investigation into ACC technology is warranted for potential integration into official standard methods for dairy product examination.
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