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Published on: August 4, 2017
Micropropagation of gum karaya (Sterculia urens) by adventitious shoot formation and somatic embryogenesis
V G Sunnichan1, K R Shivanna1, H Y Mohan Ram2
1Department of Botany, University of Delhi, Delhi 110 007, India, , , , , , IN.
Abstract:
Nodal explants from selected trees of gum karaya (Sterculia urens Roxb.) in the adult growth phase cultured on Murashige and Skoog (MS) medium supplemented with 6.62 μM N6-benzylaminopurine (BAP) produced an average of six adventitious shoots in 30 days. Shoots were rooted in vitro on 1/4-strength MS medium containing 9.82 μM indole-3-butyric acid. Nodulated callus was produced from hypocotyl explants cultured on MS medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid and 8.90 μM BAP. Somatic embryos developed when the nodulated callus was transferred to MS medium containing 0.45 μM thidiazuron (TDZ). TDZ treatment for 2 days gave the optimum response. Over 30% of the somatic embryos developed into plantlets when transferred to 1/4-strength MS basal medium without any growth regulators. Plantlets produced from adventitious shoots and somatic embryos were acclimatized to ex vitro conditions and established in the field.
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